%0 Journal Article
%T Assessment of the Biosafety of Microorganisms and their Joint Composition
%A Albina Vladimirovna Luneva
%A Yury Andreevich Lysenko
%A Anna Nikolaevna Gneush
%A Aliy Yusufovich Shantyz
%A Alexander Nikolaevich Simonov
%A Marina Nikolaevna Verevkina
%A Sergey Nikolaevich Povetkin
%J Pharmacophore
%@ 2229-5402
%D 2021
%V 12
%N 3
%R 10.51847/M60cnxYHzx
%P 42-48
%X This publication presents the results of studying the biosafety of microorganisms used for the development of a biological product-a litter destructor, both individually and in a joint composition. In the experiment, the culture of Stylonychia (Stylonychia mytilus) and laboratory animals (rabbits, mice, and rats) were used. Based on a series of preclinical studies, it was found that the studied microorganisms individually do not indicate any symptoms of virulence, toxigenicity, toxicity and do not give an affirmative counteraction to the formation of hemolysin. The results of the express method showed that the aqueous and acetone extracts obtained from the joint microbial composition did not have a detrimental effect on the culture of protozoa, the viability in all groups was 100.0%. The analysis of the dermonecrotic effect of the studied microbial preparation demonstrated that the studied extracts did not cause hemorrhage, hyperemia, peeling, edema, and other pathology indicating an inflammatory process on the skin of laboratory rabbits. Conducting an acute experiment confirmed the safety of the studied microbial consortium, since during the research the laboratory mice remained active, mobile, no signs of poisoning or other pathology were found, and the conducted pathoanatomical autopsy did not reveal lesions of the parenchymal organs of the laboratory test objects. In general, the results of the studies indicated that the investigated cultures of microorganisms and their joint composition in the composition of the biological product-destructor do not show a toxic impact on the body of lab test objects, which confirms their harmlessness.
%U https://pharmacophorejournal.com/article/assessment-of-the-biosafety-of-microorganisms-and-their-joint-composition-6gfaek15wdsoegk