%0 Journal Article
%T Impact of Growth Stages on Phytoconstituents, HPLC Analysis and Invitro Biological Activity of Rumex Vesicarius Grown in Rafha, Saudi Arabia
%A Naira Nayeem Akmal
%A Mohd. Imran
%A Samreen Soomro
%J Pharmacophore
%@ 2229-5402
%D 2023
%V 14
%N 3
%R 10.51847/nkQiIJ5yE6
%P 15-22
%X Rumex vesicarius belongs to the family Polygonaceae and is a traditional herb used for the treatment of various diseases and disorders. The current research was focused on evaluating the influence of the stage of plant growth on phytochemistry and biological activity. The hydroalcoholic extracts of frontal and mature leaves were evaluated for total phenolic acid by the Folin ciocalteu method and flavonoid content by the Aluminum chloride method. The physicochemical parameters like ash values, extractive value, amount of moisture, loss on drying, and crude fibers were evaluated using standard methods. The antioxidant activity was estimated by 1, 1-Diphenyl-2-picrylhydrazyl and Hydrogen Peroxide Scavenging method. HPLC study was carried out using gallic acid and quercitin as biomarkers. Total phenolic acid and total flavonoid content were more in mature leaf i.e., 290.66±0.85 μg/ml and 16.33 ±0.70. HPLC investigation exhibited more area under the curve for quercitin and gallic acid in the mature leaf.  The extract of the mature leaf exhibited good antioxidant activity when compared to the frontal leaf extract. The variance in the activity was credited to the stages of development which play a key role in the production of plant metabolites. This could aid in ascertaining the best growth stage for harvesting the plant.
%U https://pharmacophorejournal.com/article/impact-of-growth-stages-on-phytoconstituents-hplc-analysis-and-invitro-biological-activity-of-rumex-23ru68drnv4qotz