%0 Journal Article
%T Isolation and Purification of Superoxide-Producing Protein Complex from Helianthus tuberosus, Daucus sativus, and Solanum tuberosum
%A Ruzanna Simonyan
%A Madlena Babayan
%A Hasmik Yekmalyan
%A Anna Alexanyan
%A Gegham Simonyan
%A Samvel Alexanyan
%A Lilit Darbinyan
%A Karen Simonyan
%A Maxim Simonyan
%J Pharmacophore
%@ 2229-5402
%D 2023
%V 14
%N 5
%R 10.51847/XdwSHDmxQr
%P 107-111
%X In this study, isoforms of NPC-Fe(III) complexes were isolated and purified from Armenian Helianthus tuberosus, Solanum tuberosum, and Daucus sativus. The physicochemical properties of these complexes were determined for the first time, including specific amounts, О2--producing activity, stationary concentration of О2-, and fluorescence intensity. The complexes exhibit maximal optical absorptions in the visible and UV regions. Hybrid associates between the isolated NPC-Fe(III) complexes and NADPH oxidase (Nox) were formed, and the production of О2- by these hybrid associates was observed. The complexes continuously produce О2- in aerobic conditions by utilizing electrons from NPC, transferring them to Fe(III), and subsequently reducing О2 to О2-. Finally, О2 stabilizes О2-.

Overall, these results suggest that the isoforms of NPC-Fe(III) complexes represent new prooxidant components in the aforementioned sources and that NPC serves as a bioelectric  source for the production of О2-.

 
%U https://pharmacophorejournal.com/article/isolation-and-purification-of-superoxide-producing-protein-complex-from-helianthus-tuberosus-daucus-vgq5mspj0pmyiwi