TY  - JOUR
T1  - Isolation and Purification of Superoxide-Producing Protein Complex from Helianthus tuberosus, Daucus sativus, and Solanum tuberosum
A1  - Ruzanna Simonyan
A1  - Madlena Babayan
A1  - Hasmik Yekmalyan
A1  - Anna Alexanyan
A1  - Gegham Simonyan
A1  - Samvel Alexanyan
A1  - Lilit Darbinyan
A1  - Karen Simonyan
A1  - Maxim Simonyan
JF  - Pharmacophore
JO  - Pharmacophore
SN  - 2229-5402
Y1  - 2023
VL  - 14
IS  - 5
DO  - 10.51847/XdwSHDmxQr
SP  - 107
EP  - 111
N2  - In this study, isoforms of NPC-Fe(III) complexes were isolated and purified from Armenian Helianthus tuberosus, Solanum tuberosum, and Daucus sativus. The physicochemical properties of these complexes were determined for the first time, including specific amounts, О2--producing activity, stationary concentration of О2-, and fluorescence intensity. The complexes exhibit maximal optical absorptions in the visible and UV regions. Hybrid associates between the isolated NPC-Fe(III) complexes and NADPH oxidase (Nox) were formed, and the production of О2- by these hybrid associates was observed. The complexes continuously produce О2- in aerobic conditions by utilizing electrons from NPC, transferring them to Fe(III), and subsequently reducing О2 to О2-. Finally, О2 stabilizes О2-.

Overall, these results suggest that the isoforms of NPC-Fe(III) complexes represent new prooxidant components in the aforementioned sources and that NPC serves as a bioelectric  source for the production of О2-.

 
UR  - https://pharmacophorejournal.com/article/isolation-and-purification-of-superoxide-producing-protein-complex-from-helianthus-tuberosus-daucus-vgq5mspj0pmyiwi
ER  -