The flowers of N. nouchali were collected from lakes in Thane district dried, powdered and extracted with Pet ether, toluene, chloroform, acetone, ethyl acetate and methanol. These crude extracts were tested for antibacterial activity by agar streak method, the extracts found to be active were subjected to Minimum inhibitory concentration (MIC) determination by TTC assay, the extracts were prepared according to the MIC and antibacterial susceptibility test was carried out using Agar well diffusion method. All the extracts showed antibacterial activity against the tested strains. But the polar extracts showed a greater antibacterial potential as compared to the non polar extracts. Methanolic extract was the most active. The highest activity was seen against Salmonella paratyphi A and the lowest activity was seen against Salmonella paratyphi B. The present study showed the effectiveness of the crude plant extract against the tested bacterial strains and indicates the potential use of the extract as antimicrobial agent for the control of infectious diseases.