The present study has been designed to evaluate the antimicrobial and cytotoxic activities of crude aqueous and methanolic extracts of Incarvillea emodi along with its active polyamide column fractions. The in-vitro cytotoxic activity of two polyamide column fractions was performed by CVV assay method against CHOK 1 (Chinese hamster ovary cell line). The crude aqueous and methanolic extracts (1, 2, 4, 8, 16, 32, 64, 128, 256, 512 and 1024 μg/ml concentration) of I.emodi were taken for antimicrobial activity against four bacteria and three fungi. Effect of inhibition of cell growth by two polyamide column fractions showed significant cytotoxicity against CHO-K1 with PGI 94.1 % and 93.89 %. The results obtained from the study indicate comparatively good antifungal activity than antibacterial activity. The present study concluded that the aqueous and methanolic extracts and two polyamide column fractions of Incarvillea emodi possess potent antimicrobial and cytotoxic activities respectively. Further investigations are in progress to isolate pure compounds from the two active polyamide column fractions and their cytotoxic effects against CHO-K1 cell line.