Pharmacophore an International Research Journal
A simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of Albuterol (AB) in human plasma by using the solid phase extraction method. Amlodipine (AD) was used as an internal standard (IS). Chromatographic separation was achieved on inertsil ODS C 18 (4.6 mm×150mm,5μ) column with an isocratic mobile phase composed of 0.1% formic acid and Acetonitrile in the ratio of 10:90(v/v),at a flow rate of 0.6ml/min. AB and AD were detected with proton adducts at m/z 240.2-148.4 and m/z 409.2-238.1 in MRM positive mode respectively. The method was validated over a linear concentration range of 10.5-4119.760 pg/ml with a correlation coefficient (r2) ≥ 0.990. This method demonstrated intra and inter day precision within 5.4-9% and 4.1-9.3% and accuracy within 95.7-104% and 97.7-103.4%.AB was found to be stable throughout three freeze thaw cycles, bench top ,short term and long term stability studies.
