TY  - JOUR
T1  - DEVELOPMENT AND VALIDATION OF REVERSE-PHASE HPLC METHOD FOR ESTIMATION OF KETOCONAZOLE IN BULK DRUG
A1  - Rakesh Kumar Jat
A1  - S Sharma
A1  - R.C. Chhipa
A1  - Rambir Singh and Imran Alam
JF  - Pharmacophore
JO  - Pharmacophore
SN  - 2229-5402
Y1  - 2012
VL  - 3
IS  - 2
SP  - 123
EP  - 129
N2  - A simple, accurate rapid and precise RP-HPLC method has been developed and validated for determination of ketoconazole in bulk drug. The RP-HPLC separation was achieved on Promosil C-18, (250 mm, 4.6 mm, 5μm) using mobile phase water : acetonitrile : buffer ph 6.8 (51:45:4 v/v) at flow rate of 1.0 ml/min at ambient temperature. The retention times were 2.713 min. for ketoconazole. Calibration plots were linear over the concentration range 1-50μg/ml. Quantification was achieved with photodiode array detection at 238 nm over the concentration range of 1-50 μg/ml. The method was validated statistically and applied successfully for the determination of ketoconazole. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for the routine determination of ketoconazole in bulk drug.
UR  - https://pharmacophorejournal.com/article/development-and-validation-of-reverse-phase-hplc-method-for-estimation-of-ketoconazole-in-bulk-drug
ER  -