Archive \ Volume.11 2020 Issue 1

Development and validation of simultaneous HPLC method for determination of Lidocaine, Hydrocortisone Acetate and Methyl Paraben in Anti Hemorrhoid ointment

Seyed Majid Sedighi Nalkiashary , Mahshid Nikpour Nezhati , Homayon Ahmad Panahi
Abstract

The present study is aimed to develop a simple, rapid, sensitive and precise high performance liquid chromatographic (HPLC) method for the simultaneous determination of Lidocaine, Hydrocortisone Acetate and Methyl Paraben in Anti Hemorrhoid ointment. This research was carried out by employing an Inert sil ODS C18 column, 5 μ, 25cm × 4.60mm id with a flow rate of 1 ml/min, using a mobile phase combination of  Methanol and phosphate buffer (PH 8) in a ratio of 65:35 V/V. Detection was carried out at 230 nm. The retention time of Lidocaine, Hydrocortisone Acetate and Methyl Paraben was found to be 3.93 min, 1.26min, and 7.03 min respectively. The developed method is simple, precise and reproducible. Hence the proposed method can be used for the simultaneous determination of Lidocaine, Hydrocortisone Acetate and Methyl Paraben in Anti Hemorrhoid ointment‎‎‎‎.


How to cite:
Nalkiashary S M S, Nezhati M N, Panahi H A. Development and validation of simultaneous HPLC method for determination of Lidocaine, Hydrocortisone Acetate and Methyl Paraben in Anti Hemorrhoid ointment. Pharmacophore 2020;11(1):35-42

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