%0 Journal Article %T METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF ORLISTAT BULK DRUG USING HYDROXYLAMINE SOLUTION BY VISIBLE SPECTROSCOPY %A Alekhya Prasanna %A Suman Pattanayak and K Padmalatha %J Pharmacophore %@ 2229-5402 %D 2016 %V 7 %N 1 %P 28-34 %X A simple, precise, accurate visible spectroscopic method was developed and validated for the determination of Orlistat by using Hydroxylamine reagent and ferric chloride solution in co-solvent ethanol and subsequently diluted with distilled water. At the λmax of 515 nm, it was proved linear in the range 5-15 μg/ml, and exhibit good correlation coefficient (R2 =0.9978) and excellent mean recovery (98.82-102.3% w/w). The developed method was validated for accuracy, precision, robustness, ruggedness according to ICH guidelines. All these parameters showed adaptability of the method for the quality control analysis of Orlistat API. %U https://pharmacophorejournal.com/article/method-development-and-validation-for-the-estimation-of-orlistat-bulk-drug-using-hydroxylamine-solutionby-visible-spectroscopy