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Open Access | Published: 2014 - Issue 2

STABILITY INDICATING HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN AND PHENYLEPHRINE IN PHARMACEUTICAL DOSAGE FORM Download PDF


Khushbu B. Patel, Krupa C. Thula and Dilip G Maheshwari
Abstract

A simple, specific, accurate, and stability indicating reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of ciprofloxacin hydrochloride and phenylephrine hydrochloride using a Zorbax Bonus RP C18 column and a mobile phase composed of Water : Acetonitrile : Triethylamine (85: 15: 0.1, v/v/v), pH 3 adjusted with orthophosphoric acid. The retention times of ciprofloxacin hydrochloride and phenylephrine hydrochloride were found to be 3.71 min and 2.17 min, respectively. Linearity was established for ciprofloxacin hydrochloride and phenylephrine hydrochloride in the range of 150-900 μg/ml and 5-30 μg/ml, respectively. The percentage recoveries of ciprofloxacin hydrochloride and phenylephrine hydrochloride were found to be in the range of 98.04-101.04%. Both the drugs were subjected to acid and base hydrolysis, oxidation, UV and thermal degradation conditions. Degradation peak was well resolved from the main peak of drug. This method can be successfully employed for simultaneous quantitative analysis of ciprofloxacin hydrochloride and phenylephrine hydrochloride in bulk drugs and formulations.

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Pharmacophore
ISSN: 2229-5402

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