Introduction: The aim of this study was to determine the relationship between job burnout and spiritual intelligence of nurses. Methods: This is a cross-sectional study that was conducted in 2017 in educational hospitals of Jahrom University of Medical Sciences. 100 nurses were selected randomly. Maslach Burnout Questionnaire (1985) has been used to measure job burnout. The questionnaire is a 22-item Likert questionnaire and its severity is from very little to very much. The formal and content validity of the instruments was examined by 5 university professors and its reliability was calculated by using Cronbach's alpha with the help of 10 nurses for emotional exhaustion, depersonalization and personal adequacy (0.94 _ 0.93_ 0.91), respectively. Spiritual intelligence questionnaire Abdollahzadeh was used to investigate spiritual intelligence. The formal and content validity was obtained with the help of five professors and its reliability was obtained 0.89 by using the Ray test. Data was analyzed by using SPSS19 software. Results: By using linear regression, it was found that the whole average of spiritual intelligence could predict exhaustion, depersonalization, and individual adequacy. Spiritual intelligence subgroups were able to predict emotional exhaustion, depersonalization, and individual adequacy. The average score of total spiritual intelligence can predict the emotional exhaustion, depersonalization, and individual adequacy variations. Also, sub-groups of spiritual intelligence could predict variations in emotional exhaustion, depersonalization, and individual adequacy. Conclusion: Spiritual intelligence has indirect relationship with job burnout so that people with the high spiritual intelligence have better performance and lower job burnout.