Introduction: Assessment of students' perception of educational and learning environment is one of the tools for Knowing the quality of education, promoting and modifying the curriculum, this study aimed to assess the perception of learning environment from the viewpoints of undergraduate students in the operating room of the University of Medical Sciences. Method: This is a descriptive study that was carried out in Iran University of Medical Sciences in 2016-2017. The number of 143 undergraduate students compeleted the DREEM questionnaire.The questionnaire has 50 items that range from 0 to 200.The DREEM questionnaire has five areas: students' perception of learning,teachers,academic ability, atmosphere of education, and the social conditions of education. Results: In this study, the total score of the perception of the learning and teaching environment for the undergraduate students in the operating room. It was obtained 76 that reflects the student's view of their learning environment. There was a significant relationship between male and female students' score of 0 (P = 0.029). Male students had a more positive attitude toward female students. Also, there was a significant difference between the graded and discontinuous undergraduate student scores. The viewpoint of graduate students from undergraduate students (continuously evaluated more positively). (P=0, 01) Conclusion: The score of perception of the learning and teaching environment for undergraduate students of the operating room of the University of Medical Sciences indicates that students' perceptions of their learning environment are desirable. The perception of discontinuous undergraduate students was steadily more positive than undergraduate students.