Calendula officinalis effects on expression bFGF and TGFβ1 in fibroblasts Download PDF

Background: Calendula officinalis L. belongs to the asteraceae family which has various components such as triterpenes, saponins, tannins and volatile oils and are used for medicinal purposes like dermatitis and wound healing, but the mechanism of this effect is not fully known. Scince TGFβ1 and bFGF are two important factors that are expressed in fibroblast cells. Our study aim to evaluate the effect of methanolic extract of Calendula officinalis on the proliferation and expression of angiogenesis growth factors (TGFβ1 and bFGF) in mouse embryonic fibroblast cells. Methods: The cells were exposed to different concentrations of methanolic extract of Calendula officinalis at different times and cell proliferation and expression of TGFβ1and bFGF were measured at the level of gene and protein by real time-polymerase chain reaction (real-time-PCR) and enzyme-linked immunoslobent assay (ELISA) respectively. Results: The results showed that Calendula officinalis extract were not toxic for cells in study concentrations and increased gene expression of TGFβ1 and bFGF after 12 hours, but after 24 hours, the expression of these genes were down-regulated. Conclusion: The results of research showed that changing patterns of expression TGFβ1 and bFGF were similar and the level of changes depend to dose and time.