A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Irbesartan in tablet dosage form. An Inertsil ODS C-18, 5μm column having 250 x 4.6mm internal diameter in isocratic mode with mobile phase containing methanol, acetonitrile and 2% OPA (40:40:20,v/v/v) was used. The flow rate was 1.5 ml/min and effluents were monitored at 260 nm. The retention time for Irbesartan was 4.5 min. The method was validated for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of detection and limit of quantification were found 10 ng and 30 ng respectively and recovery of Irbesartan from tablet formulation was found 100.61%. The proposed method was successfully applied for the quantitative determination of Irbesartan in tablet formulation.