Pharmacophore an International Research Journal
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Open Access | Published: 2011 - Issue 3

DEVELOPMENT AND VALIDATION OF LC METHOD FOR THE ESTIMATION OF ZOLMITRIPTAN IN PHARMACEUTICAL DOSAGE FORM Download PDF


N.Usha Rani, R. Sreenivasa Rao, K .Saraswathi, T.E.G.K. Murthy
Abstract

A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Zolmitriptan in tablet dosage form. A chromosil C-18, 5μm column having 250 x 4.6 mm internal diameter in isocratic mode with mobile phase containing acetonitrile: methanol: orthophosphoric acid in the ratio of 69:29:02 (v/v/v) was used. The flow rate was
1.0 ml/min and effluents were monitored at 210 nm. The retention time for Zolmitriptan was 1.9 min. The method was validated for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of detection and limit of quantification were found to be 0.005 ppm and 0.0165 ppm respectively and recovery of Zolmitriptan from tablet formulation was found to be 99.85%. The proposed method was successfully applied for the quantitative determination of Zolmitriptan in tablet formulation.

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Pharmacophore
ISSN: 2229-5402


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