Pharmacophore an International Research Journal
This study is aimed at developing a simple UV-visible Spectrophotometric method for determination of curcumin in its pure form and pharmaceutical formulations to validate the developed method. Curcumin was estimated using UV-Visible double beam spectrophotometer at absorption maxima of 429 nm in solvent system containing pH 7.4 phosphate buffer and ethanol in mixture (1:1). The analytical parameters like linearity, precision, and accuracy, by following International Conference on Harmonization (ICH) guidelines were determined. The developed method showed linear response for concentration range of 2-10 µg/ml of curcumin and according to Beer’s law, a coefficient of correlation of 0.998 was found. The accuracy was between 99.79 and 100.27. The limit of detection (LOD) and limit of quantification (LOQ) were determined for the sensitivity of the method, which were found to be 0.861 µg/ml and 2.872 µg/ml, respectively. The study revealed that the method for estimation of curcumin is linear, accurate, precise and economical and further can be used for testing the pharmaceutical formulations.
