Cancer has abnormal cell growth in the body that can lead to death. For thousands of years, plants have been used to cure many diseases. Given the prevalence of breast cancer in Iran, the ascending tilt of this malignancy over the past two decades, high mortality rates and on the other hand, with regard to oral intake, cheap and easy access by the general public to the extract of the milk thistle the effectiveness of this herbal product was investigated on MCF-7 breast cancer cells. Also, in recent decades, scientists have used natural compounds to treat cancer, including raw vegetable extract or a combination of different phytochemicals. Study on cytotoxic activity of raw ethanol extracts from the plant Euphorbia maculate strain Euphorbiacea on the MCF-7 cell line. One of the most valuable biological activities in these species is anti-cancer. In summary, the results of the research confirm the effectiveness of these plants against breast cancer and given the nature of the product, its low cost and public access, entering this herbal supplement in the diet may be effective in treating breast cancer. Based on the results of the extract of E. maculata L. Has a poor inhibitory effect on MCF-7 cells.