Eugenol is extensively used in dentistry as analgesic and anti-inflammatory. Due to its small size and high density of sensory nerves, cornea is one of the most sensitive tissues of the body. Major sensory nerves of the cornea are poly-modal pain nerves, which respond to thermal, mechanical, or chemical stimuli. The aim of this study was to determine effects of the topical eye and intraperitoneal administration of eugenol on acute corneal pain in male rats. In this experimental study, Seventy-two male Wistar rats were randomly divided into 9 groups of 8 as follows: two topical and intraperitoneal control groups that received normal saline via corneal surface and intraperitoneal ways respectively. The positive control group received 3.5 mg/kg morphine. Three groups received eugenol via corneal surface and three groups intraperitoneally at doses of 3, 10, and 30 mg/kg. Forty µl of 5 M sodium chloride was used to induce corneal pain. One hour after drug administration, the number of eye rubbing in 30 seconds was used to measure acute corneal pain. Morphine significantly reduced eye rubbing caused by 5M sodium chloride. Different amounts of topical ocular and intraperitoneal eugenol significantly decreased acute corneal pain. Topical ocular concentration of 10 and intraperitoneal concentration of 30 showed the best analgesic effect. The analgesic effects of eugenol on acute pain of cornea can be considered in future research.