Effects of topical application of Plantago major leaf alcoholic extract on excisional wound healing in BALB/c mice Download PDF

Objective: The aim of this study was to evaluate the effects of Plantago major L. leaves ethanol extract (pm) on excision wound healing in BALB/c mice. Method: sixty male BALB/c mice (2.5 months of age) were divided into two experimental and three control groups (n=12).Under anesthesia, two circular excisional wounds (5mm diameter) were made on dorsal thoracic area of the mice skins by a disposable surgical punch. The experimental groups received topical cream of PM at the concentrations of 5% and 10% while control groups treated with nitrofurazone cream 1% (positive control), cold cream (negative control) or no treatment (sham control). On the days 4, 7, 10 and 14 after treatments, excisional biopsies were performed and wound healing was evaluated histopathologically. Results: Compared to the negative control, Total inflammatory cells significantly decreased in both of the experimental groups at the days 4, 7 and 10. The proliferation phase markers including the number of fibroblast cells and granulation were significantly higher in experimental groups than negative control group. Assessment density of collagen fibers between groups showed that PM in a dose dependent manner significantly improved collagen synthesis.