Three different extracts of Chromolaena odorata root bark were tested for their total phenolic contents, total antioxidant activity, DPPH, ferric reducing activities (Fe3+- Fe2+) and H2O2 scavenging activity. Total phenolic contents (TPC) were high in CoEAE when compared with CoMeE and CoAqE. In redox potential assay methods CoMeE showed maximum total antioxidant activity, DPPH activity (IC50) and ferric reducing activity when compared with CoEAE and CoAqE. Hydrogen peroxide decomposing capacity was higher in CoMeE (IC50) when compared with CoEAE and CoAqE. The phytochemical screening revealed the presence of saponins, tannins, triterpenoids, and phenols are common in both extracts, alkaloids and carbohydrates were present only in CoMeE. The scientific experimental investigations of the present study revealed that potential antioxidants (may be phenolic, flavonoids, tannins, saponins and triterpenoids) present in CoMeE and CoEAE can be used against the free radical associated degenerative diseases. But proper toxicity evaluation is needed to proceed further for clinical therapeutic use.