Citrus grandis Osbeck provides juicy, tasty and nutritious fruit with aesthetic qualities for human beings. The phytochemical composition was determined and the in vitro antimicrobial activities of leaf, stem, stem bark and ripe fruit peel extracts of C. grandis were evaluated using standard techniques. The signiﬁcance of the results was evaluated using Duncan’s multiple range test. The highest contents of alkaloids (2.76±0.06 mg/100g), flavonoids (3.41±0.12 mg/100g), phenols (1.64±0.11 mg/100g), sterols (0.82±0.03 mg/100g) and tannins (3.46±0.06 mg/100g) were detected in the peel. The peel and stem bark extracts had the lowest MIC values against Staphylococcus aureus, Escherichia coli, and Rhizopus stolonifer. This study disclosed that the extracts of C. grandis parts are rich with phytochemicals that are active against fungi and bacteria, hence, suggesting its pharmacological significance.