This study aimed to formulate and evaluate nasal drug delivery system containing Salbutamol Sulphate for improving the bioavailability and sustaining the drug release. Salbutamol Sulphate is a selective β2 adrenoreceptor agonist and rapidly absorbed from gastro intestinal tract but it is subjected to first pass metabolism. Thus oral bioavailability is only 50%. Hence, an In situ nasal gel of Salbutamol Sulphate was prepared to enhance its bioavailability as well as reducing the dosing frequency. Natural polymer and its carboxymethyl derivative were used to enhance mucoadhesive and poloxamer 407 was used for its sol gel property. DRG was successfully derivatized to CMDRG by etherification reaction. Drug and polymers were characterized by FTIR and DSC analysis. Salbutamol Sulphate was selected for formulation of in situ gel. DRG and CMDRG were used in varying concentration as a mucoadhesive polymer. The result revealed that as the Increase in concentration of polymers should decrease in gelation temperature of formulations while increase in concentration of polymers produced increase in mucoadhesion force of all formulations. pH of all formulations were in the range of 5.3 and 6.5. The drug content of all formulation was found to be 93.6% - 96.66%. The drug release was extended up to 8 hrs.