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Open Access | Published: 2019 - Issue 6

Genetics Identification and Detection of L-Methioninase Gene In a Pseudomonas Isolate Download PDF

Al-Zahrani S.H.M., Bukhari K.A.

Cancer is a growing cause of death and morbidity worldwide. L-methioninase (EC has a potential application against many types of cancers like glioblastoma, kidney, breast, lung, and colon cancer. The present study aimed to screen microbial production of L-methioninase from marine samples obtained from Jeddah coast In Saudi Arabia. Sixteen bacterial isolates were obtained from different marine samples. The bacterial isolate's ability for L-methioninase production was tested on a modified M9 L-methionine agar medium using phenol red as the pH indicator. Colonies with pink-red and yellow zone around were selected as L-methionine degrading bacteria. Results revealed the incubation of the cultures in a shaking incubator (150rpm) led to decreased L-methioninase production by some isolates but increased the creation of the enzyme by other isolates after 48 hours of incubation. The highest L-methioninase producing isolate (Pseudomonas extremaustralis) was obtained from a seashell, and identified based on 16S rDNA sequencing, along with the biochemical characterization, BLAST analysis, and the structure of a phylogenetic tree. The rRNA sequence of P. extremaustralis was deposited in GeneBank (NCBI accession number MK072729)‎‎‎‎.
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ISSN: 2229-5402

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