Histomorphometric and histological changes of testis in busulfan-induced oligospermia and azoospermia infertility Download PDF

Aim: The objective of the study was to determine the histological and hormonal changes in busulfan-induced oligospermia and azoospermia. Materials and Methods: Eighteen BALB/c mice were divided into three equalgroups. Oligospermic group received a dose of 10 mg/kg busulfanintraperitoneally, the azoospermic group received a second dose of busulfan similarly, 21 days after the first injection and the control groupreceived no busulfan.All animals were assessed for histologic, histomorphometric and hormonal changes of testosterone and anti-Mullerian after 35 days. Results: In an azoospermic group, the luminal diameter and area significantly increased in comparison to oligospermic animals and this increase was less than the control group. In an azoospermic group, cellular and total diameters and cellular and cross-sectional areas significantly decreased in comparison to oligospermic group and this decrease was less than the control group. Numerical density and tubular count per area unit in the azoospermic group were significantly more than the oligospermic group and in both groups were more than the control group. In mice receiving busulfan, spermatogenesis index significantly decreased when compared to the control group. In an oligispermic group, the testosterone levelwassignificantly more than other groups while anit-Mullerian level showed no changes. Discussion: Histomorphometric, histological and hormonal assessment were introduced as prominent variables to confirm successful induction of oligo- and azoospermia,while these findings can be added to the literature and open a new window for future interventions in treatment of male infertility.