Hydroxy-methyl-furfural inhibits secretion of selected inflammatory fac-tos in a coculture system of HUVECs with human monocytic cell lines Download PDF

Objectives. There is sufficient evidence that bioactive compounds in foods may reduce level of persistent inflammation, a key contributor to development of atherosclerosis. This study was sought to determine whether exposure to Hydroxymethylfurfural (5-HMF) diminishes levels of ICAM-1 and MMP-9 from coculture of Human umbilical vein endothelial cells(HUVECs) with human monocytic cell. Design. A co-culture system was established between HUVECs and human monocytic cell line (THP-1). After treatments of the cocltivated cells with different concentrations of HMF, the viability of cells in the system was investigated using MTT assay. Also, the anti-inflammatory responses of the system was studied by measuring the levels of ICAM-1 and MMP-9 in supernatants by ELISA. Results. It was found that treatment with 5‐HMF significantly decreased MMP-9 level (P<0.05) with no significant effect on secretion of ICAM-1. Furthermore, HUVECs cell ‘s viability viability showed equal or decreasing tendency, but coculture cell viability increased with increasing the dose of 5-HMF (p<0.05). Conclusion. Our data confirms the anti-inflammatory properties of 5-HMF. The finding of this study could be applicable in prevention and management of atherosclerosis.