Methanol, chloroform and hexane extracts from leaves of Kirganelia reticulata, used in Indian ayurvedic medicine for the treatment of several ailments of microbial and non-microbial origin were evaluated for potential antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). Antibacterial activity and biofilm production of crude extracts against MRSA (ATCC 25923) isolated from clinical specimen was studied. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the methanol, chloroform and hexane extracts were in the range of 12.5 to 50.0 mg/ml and 25.0 to 100.0 mg/ml, respectively. Amongst the evaluated extracts, the methanolic extract showed the strongest antibacterial effect as well as biofilm inhibition. Micro plate screening used for detection of biofilm formation by Staphylococci is a quantitative model to study its adherence level and has been a sensitive method.