Investigation of the Effect of Royal Jelly on Amount of Nitric Oxide in Ovariectomized Rats Download PDF

Aim: Epilepsy is a chronic and neurological disorder that leads to abnormal and unconscious activities in person. epilepsy can have some Risk factors such as hypoglycemia and hypoxia, head injury, CNS infection of brain tumors, oxidative stress, inflammation of the brain and increase in nitric oxide. Menopause is one of the physiological conditions that, producing cytokines such as IL-1 β, can cause to increase the brain inflammation and be the ground of neurological diseases and seizure. In recent years, the drugs reducing the inflammation of the brain have attracted attention for treating epilepsy and seizure. Royal Jelly is a slimy substance produced by worker bees. This contains a different material that has abundant biological activities such as an increase in neuronal differentiation and neurogenesis. Its most important attribute of interest is the antioxidant and anti-inflammatory activity of this substance against nitric oxide, active oxygen species and oxidative stress. The aim of this study was to investigate the effect of Royal Jelly on the nitric oxide caused by epilepsy induced by pentylenetetrazol (PTZ) in ovariectomized rats. Material and Method: 80 rats were divided into experiment and control groups and fallen under treatment with royal jelly. 24 hours after the last injection of Royal Jelly, PTZ was injected by 80 mg/kg in intraperitoneal manner in order to make seizure and the behavior of rats was studied and evaluated for two hours and then the rats’ brain was dissected and sent out of the skull. Histological cuts were done by using the microtome in CA1 region of Hippocampus and the cresyl violet coloration was used for showing the nerve cells. Measurement of nitric oxide was done by the Griess reaction with the microplate method. After the reaction and formation of color the optical absorption resulting from forming colorful matter in wave length of 540 nm was read by reader device Elisa. Results: There was not observed any significant difference in the amount of measured nitric oxide at any of the different groups under study. While there was observed a significant difference in total amount of antioxidants and the number of neuronal cells and in all the groups under study after treatment with Royal Jelly. Discussion: The results of this study showed that treatment with Royal Jelly has been not effective in reducing the amount of nitric oxide in the epilepsy induced by pentylenetetrazil in ovariectomized rats. This is while treatment with Royal Jelly increased the number of neuronal cells and the amount of antioxidants in rats with seizure. So it can be claimed that the Royal Jelly can be applied for reducing epilepsy effects.