Isolation and Purification Commercial Aerolysin Enzyme from Aeromonas Hydrophila in Natural Flora of Gut System in Warm Water Fish
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Toktam Abbasnia1, Ahmad Asoodeh2, Gholamreza Habibi3, Alireza Haghparast1, 4*
Abstract
An important pathogenic factor for bacteria is secretory toxins. Aerolysin is a virulence factor of A.hydrophila. hemolytic activity of aerolysin destroys the cell membrane of eukaryotic cells by pore-forming. The receptor of this enzyme is GPI-anchored proteins. These two properties (hemolytic activity, GPI-anchored protein receptor) caused used aerolysin for different goals. There is recombinant form of aerolysin commercially but this is very expensive. This study determines a simple method for aerolysin isolation from A.hydrophila.
