Ficus species is one of the most important medicinal sources of nature with effective properties to treat various diseases. In the present study, an attempt had been made to isolate, purify and characterize bioactive compounds from Ficus krishnae. Stem bark sample of Ficus krishnae subjected for extraction with methanol and dried the obtained extract. Separation and purification of terpenoids were done by thin-layer chromatography, column chromatography and preliminary phytochemical screening by Salkowski test and Liebermann Burchard’s tests. The isolated pure compound is characterized by FTIR, NMR, and GC-MS. The above spectral and physicochemical data isolated pure compound from the stem bark of Ficus krishnae was phytol [3, 7, 11, 15-Tetramethyl-2-hexadecen-1-ol] having a molecular formula of C20H40O.