This cross-sectional survey aimed to assess the Knowledge and Practices of Dental Professionals towards the Use of Lasers in Riyadh City. The study subjects comprised general dentists and specialists/consultants with experience of 1-6 years or more than 6 years. Knowledge and Practices of Dental Professionals towards the Use of Lasers were measured using an eleven-item questionnaire. After ensuring the reliability of the questionnaire, differences across gender, clinical position, and clinical experience were seen using a statistical measure Chi-square through SPSS to determine the statistically significant differences (p<0.05). Findings revealed that 75.5% of participants were male, and 24.5% were female out of the total population. 88.2% of the sample was working as general practitioners and the rest as a specialist, 90% of participants have experience of 1-6 years, and only 10% have more than 6 years of experience. Findings revealed significant differences in knowledge about laser, use of laser, for hard and soft tissues preference. Males prefer GAALAS for hard tissues, while females prefer Ruby. For soft tissues, it was GAALAS and Excimer, respectively. Specialists with experience mark satisfactory knowledge but never used laser in practice as compared to general dentists.