Pharmacophore an International Research Journal
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Open Access | Published: 2014 - Issue 3

METHOD DEVELOPMENT AND VALIDATION OF CAPROIC ACID FROM VANILLA PLANIFOLIA PODS BY HPLC Download PDF


Shruthi Menon Naira Nayeem and MK Ranganath
Abstract

The aim of the current study was to develop and validate a simple, reliable and fast HPLC method for the determination of caproic acid in the methanolic extract of Vanilla planifolia using reversed phase high pressure liquid chromatography (RP-HPLC). The column used for separation was Agilent C18 Zorbax 150 x 4.6 mm, 5μ particle size. The mobile phase used is phosphate buffer having pH of 2.45 with methanol in the ratio 40 : 60 v/v. Isocratic elution technique was used with a flow rate of 1.0 ml/min and the injection volume was 10μl. Detection was done using a PDA detector at a wavelength of 269 nm. The method was validated in terms of system suitability, accuracy, precision, specificity, linearity range and robustness. The method produced linear responses in the range of 50-150%. Hence a simple, reliable and rapid method was developed and validated for the estimation of caproic acid in the methanolic extract of Vanilla planifolia.

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Pharmacophore
ISSN: 2229-5402


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