Pharmacophore an International Research Journal
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Open Access | Published: 2019 - Issue 1

Molecular Characterization of Some Phosphate Solubilizing Microorganisms Download PDF

Ola I.M. El-Hamshary, Fardos M. Bohkari, Lolo A. Al-Aklouk, Samah O. Noor, Azhar A. Najjar

One of the most important macronutrients to plants is phosphorus, which increases the biological activities in plants. Phosphorus is found as insoluble and unavailable form for plant. Some microbes of the soil can transform insoluble phosphate shape to a more suitable shape for plants’ requirements. The current study used some microorganisms as bacteria and fungus that had the ability to convert phosphorus. Different phosphate microorganisms have been isolated, screened, identified; and the intrinsic antibiotic resistance was tested. One hundred bacterial isolates were isolated from Curcuma, Ocimum and rhizospheres of Eruca plants and tested for phosphate solubilization. The results showed nine bacterial isolates (R1B2, R2W22, R3B28, R4B31, R5C33, R6B34, R7 C35, R8 B40, and C15) out of 100 investigated bacterial isolates, which had a potential of phosphate solubilization ability based on Pikovasky (PVK) and National Botantial Research Institute Phosphate (NBRIP). The media was based on clear halo zone, and the nine isolates of the bacteria were determined using biochemical (API 20) test. Based on the results, the identified isolates of bacteria were: Enterobacter cloacae (R1B2, R2W22, R3B28, R4B31, R5C33, R6B34, R6 C35, R7 C35 and R8 B40), Klebsiella pneumonia (C15), Bacillus cereus (R2W22) and Bacillus megaterium (R3B28). We found eight selected bacteria (R1B2, R2W22, R3B28, R4B31, R5C33, R6B34, R7C35 and R8B40 and C15) which were confirmed by 16S rRNA sequence. These sequences were submitted to the NCBI GeneBank under accession number MK064178 for E. cloacae R1B2, (MK064181) for E. cloacae R4B31, (MK064182) for E. cloacae R5C33 (MK064183) for E. cloacae R6B34, (MK064184) for E. cloacae R7C35, (MK064185) for E. cloacae B40R8, MK064179 for B. cereus R2W22 and MK064180 for B. megaterium R3B28. Intrinsic antibiotic resistance test was carried out for 8 phosphate solubilizing bacteria (R5C33, R7C35, R2W22, R8B40, R3B28, and C15) using 9 antibiotics. The obtained data revealed that B. cereus (R2W22), E. cloacae (R5C33, R7C35, and R6B34), were resistant against six antibiotics. While E. cloacae (R8B40 and R1B2) was resistance against eight antibiotics. B. megaterium (R3B28) had resistance for three antibiotics. Seven isolates of fungi were isolated from soil samples and screened for phosphate solubilization. The data showed Aspergillus niger (LA) had high phosphate solubilization and identified based on 18S rRNA sequencing. The sequence data were submitted to Gene Bank. Isolates of bacteria and fungi could be used as potent bio-fertilizer for phosphate.

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ISSN: 2229-5402

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