Colorectal cancer (CRC) is a significant health issue in Saudi Arabia, with an increasing incidence and low survival rates. Previous studies have indicated a lack of awareness and knowledge about CRC and its screening methods among the population. This study aims to address the existing gap by comprehensively assessing the knowledge and awareness of CRC and its screening across all regions of Saudi Arabia. A cross-sectional study was conducted using a structured online questionnaire. The study included individuals aged 18 and above from various regions of Saudi Arabia, excluding healthcare workers, students of health majors, and those with a personal or family history of CRC. The sample size was a minimum of 384 participants, randomly selected through web-based questionnaires distributed via social media. The questionnaire collected sociodemographic data and assessed CRC awareness, knowledge, and attitudes towards screening. Data analysis was performed using SPSS software, including descriptive statistics, chi-square tests, and the calculation of knowledge scores. The study included 1095 participants, 69.7% of them were females and 30.3% were males. 76.1% of respondents have heard of this type of cancer. Only 48% have heard of screening tests to diagnose colon cancer. Generally, 39% of participants had good knowledge of CRC, 36% had moderate knowledge, and 25% had poor knowledge. The study shows that the Saudi general population had inadequate knowledge of CRC. Knowledge scores were significantly associated with age and educational level but, not with gender.