Infections with Candida species represent a large percentage of the total number of fungal infections in the vaginal mucosa. Conventional treatments with antifungal medicinal substances have proven to be low in efficiency, so we are currently looking for alternative products based on plant extracts (these being more effective according to existing studies but also non-toxic). Our study aimed to obtain the oil of Origanum vulgare L. and its characterization from the point of view of antimicrobial activity, known for its rich composition in active principles such as carvacrol (a compound with proven antifungal activity). Also, the plant Origanum vulgare L. was characterized from a macro- and microscopic point of view. The oregano oil obtained was included in the liposomal formula and characterized from the point of view of appearance, surface electric charge, and size, respectively. The liposomal oil was also included in the formula for the preparation of vaginal suppositories. These were characterized organoleptically and from the point of view of the release of active principles. The future perspectives are to test the formulated pharmaceutical forms in vitro and then in vivo.