The present study deals with the various analytical techniques like ultra-violet spectrophotometer, high performance liquid chromatography and AxSYM system for the quality control of various vaccines. Ultra-violet spectrophotometer was used to analysis the thiomersal content in Diphtheria tetanus pertussis (DTP) vaccine 0.0107%, protein content in Rabies vaccine 0.657%, and phenol content in Diphtheria tetanus pertussis vaccine 0.230% respectively. High performance liquid chromatography was used to determine the molecular size distribution of the meningococcal vaccine 97.50% and the AxSYM system was used to estimate the in-vitro potency assay of hepatitis B 0.9769%. Formalin content in Diphtheria tetanus pertussis (DTP) vaccine 2.0% was estimated by qualitative analysis. It was confirmed that results were found after quantitative and qualitative analyzing the constituents of vaccine samples with the analytical techniques employed in the present study accurate, efficacious and rapid rather than the other previous method.