Among the heterocyclic compounds family, piperidine, as a nitrogen-containing six-membered heterocycle, is the most dominant natural element and it has often been found to be within the naturally occurring bioactive compounds like alkaloids. The derivatives of piperidin-3-one are as precursors of synthetic antimalarial agents, isofebrifugine, and febrifugine. Piperidin-4-ones have potent and varied biological properties including antitumor, herbicidal, antiviral, fungicidal, antimicrobial, insecticidal, analgesic, antihistaminic, anti-inflammatory, anticancer, and CNS stimulation, and recent investigations have shown that when aromatic substitutions are at their 2- and/or 6-positions of the compounds with a piperidin-4-one moiety have a considerable activity. In this project, N-nitroso-2,6- diphenylpiperidin-4-one semicarbazone was made and analyzed. This compound revealed a sharp melting point for purity and one spot in TLC. The compound structure was further proved by FTIR, and it was evaluated for its activity as an antioxidant against ascorbic acid (Vit C) as standard. The compound exhibited significant inhibition activities against ascorbic acid. Finally, it was docked in ArgusLab, bound to 5hmh protein which works for DNA repair, cell trafficking, etc.