The effect of aqueous extract of Origanum Vulgare leaves on Adiponectin and c-Cbl associated protein levels in adipose tissue of Streptozotocin-Nicotinamide induced diabetic rats
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Darya Ghadimi1, 2, Mohammad Taghi Goodarzi1, Nasrin Ziamajidi1, Shirin Moradkhani3, 4, Taiebeh Ghyasvand1
Abstract
Increased c-Cbl associated protein (CAP) and adiponectin level in adipocytes can facilitate insulin dependent glucose uptake in adipocytes and skeletal myocytes. Peroxisome proliferator activated receptor γ (PPARγ ) can increase the level of these proteins in adipocytes. Aqueous extract of Origanum Vulgare (OV) leaves contains Biochanin A, which is known as a PPARγ agonist. This study was designed to investigate the effect of aqueous extract of OV leaves on adiponectin and CAP levels in adipose tissue of diabetic rats. Adult male rats used as animal model in the present study. Animals were made diabetic by single intraperitoneal injection of Streptozotocin- Nicotinamide and treated by 20mg/kg of aqueous extract of OV leaves for 28 days. Fasting blood glucose (FBG) and insulin levels were tested. Furthermore, adiponectin and CAP levels in adipose tissue were examined by immunoblotting analysis. The presence of Biochanin A in aqueous extract of OV leaves was determined by high pressure liquid chromatography (HPLC) assay. In treated animals, FBG level reduced and insulin resistance index improved significantly compared with untreated diabetic rats (p<0.01). CAP and adiponectin levels in adipose tissue of treated animals increased significantly compared with diabetic and healthy control animals (p<0.01). HPLC analysis confirmed presence of Biochanin A in aqueous extract of OV leaves. Consumption of aqueous extract of OV leaves can increase adiponectin and CAP levels in adipocytes, which can lead to increased insulin dependent glucose uptake in adipocytes and skeletal myocytes.
