The production of neural stem cells as a suitable source for the treatment of neurodegenerative diseases Download PDF

Introduction: Spinal Cord Injury (SCI) is a disease that can disrupt many functions, including motor functions, today cell therapy is one of the treatments for these diseases. Adipose derived stem cells (ADSCs) from the mesenchymal stem cell group are easy and affordable access which can be differentiated into other cells. In this study, we differentiated the ADSCs by the neurospheres method into neural stem cells (NSCs). Methods: In this study, adipose tissue was taken from rats and examined after digestion in the DMEM medium and surface markers of stem cells such as CD90, 29, and 45 were examined. ADSCs were distinguished into neurospheres in a neuron culture medium and then neurospheres were converted directly to NSCs. Nestin and NF-68 neuron markers were examined by immunocytochemistry. Results: ADSCs expressed the mesenchymal stem cell markers and did not express the CD45 marker of the hematopoietic cells. Neurospheres produced from them also express the marker of neural stem cells such as nestin and NF-68 and the stem cells produced are also the same. Conclusion: Therefore, ADSCs may be an ideal alternative easily accessible source of stem cells for the treatment of neurodegenerative diseases.