Plants are the source of large amount of drugs comprising to different groups such as antispasmodics, emetics, anti oxidant, anti-cancer, antimicrobials etc. Andrographis paniculata (Burm. f.) is an herbaceous plant has been widely used for treating sore throat, flu, antioxidants and upper respiratory tract infections. Andrographolide, a major bioactive chemical constituent of the plant, has shown anticancer potential in various investigations. Andrographolide and its derivatives have antioxidants effects in in-vivo and in-vitro and reduce the free radical damage induced by chemical pollutants. Hence, the present review explains the basic properties of free radicals, free radical mediate damage and its effect on the biomolecules of the in-vivo systems. The review also give details on the phytochemical constituents of the medicinal plant Andrographis paniculata and its effect on development of antioxidants against free radical induced oxidative damage. However, what is noteworthy about this review is summarizing the effects of bioactive compounds and their role in the development of antioxidants against free radical induced oxidative damage. Therefore, this review is intended to provide evidence reported in relevant literature on qualitative research to assist scientists in isolating and characterizing bioactive compounds with more antioxidants.