The present study was executed to study the antihyperglycemic and antihyperlipidemic activity in roots related to methanol extract of Acacia suma Roxb in rats. The test was performed in rats which are alloxan-induced hyperglycemic under treatment of the tested methanol extract at 200 and 400 mg/kg dose levels for 30 days and methanol extract. glibenclamide (2.5 mg/kg) was used as a standard drug for activity comparison. The parameters related to biochemical issues used in the study are plasma-insulin, blood glucose and lipid profiles. Standard experimental method was used in performing the experiment. The results of the test showed that the blood glucose level comes down significantly (p<0.05) in normoglycemic rats in a specific does, this happened when the hyperglycemic rats experiences the sudden decrease of blood sugar (p<0.05 to 0.001). The tested dose levels of the extract, significantly (p<0.05) increases glucose by rat hemidiaphragm which is isolated. It demonstrated the remarkable antidiabetogenic effect exerted by the methanol root extract of Acacia suma.