In the case of complete or partial blockage of the passage of bile through the bile tree, a syndrome of obstructive jaundice develops. Obstructive jaundice is still a serious problem, despite the widespread adoption of auxiliary diagnostic methods. According to our observations, in 75-83% of cases, it is possible to diagnose obstructive jaundice according to clinical examination and laboratory diagnostics, but the "signs" of this syndrome have a different diagnostic value, so the purpose of the work is to determine the frequency of occurrence of certain clinical and laboratory symptoms and indicators. Materials: 427 patients aged 21-92 years with a diagnosis of "obstructive jaundice". All the patients were made a comprehensive diagnosis, including clinical and laboratory examinations. Biochemical study of blood and urine has great diagnostic value, it allows not only to assume the diagnosis but also to assess the degree of metabolic disorders due to the toxic effects of bile acids. The combination of increased total bilirubin, alkaline phosphatase activity, and gamma-glutamyltranspeptidase in our study proved to be the most sensitive for determining obstructive cholestasis and cytolysis. Nevertheless, we consider the problem of research of reliable laboratory markers of obstructive jaundice, and most importantly – markers of severity of the hepatocellular failure, to be relevant.