The objective of this study was to develop matrix tablets of levofloxacin for sustained release. Xanthan gum, guar gum, karaya gum used as natural polymers and study the effect of various formulation factors such as polymer proportion and effect of filler type on the in vitro release of the drug. Levofloxacin Matrix tablets were prepared by direct compression technique with average weight of drug of 250 mg. The prepared tablets were evaluated for weight variation, friability, hardness, thickness and in vitro dissolution studies. All the granules of formulations showed compliance with pharmacopoeial standards. From the In vitro dissolution studies it is clear that by increasing the amount of drug release is decreased as the concentration of polymer increased, drug release was found to be retarded. The formulation F7 is selected as the optimized formulation by in vitro drug release for 12 hrs with the release of 99.26%. The kinetic treatment showed that mechanism of drug tablets of levofloxacin follows non fickian transport mechanism which having n < 1 and by the stability studies there is no significant difference in the drug content. By the stability studies there is no significant difference in the drug content.