Archive \ Volume.2 2011 Issue 4

PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF AQUEOUS EXTRACT OF Phyllanthus maderaspatensis

G. Leelaprakash , S. Mohan Dass
Abstract

The phytochemical screening and antimicrobial activity of Phyllanthus maderaspatensis against a wide variety of pathogenic bacteria such as Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhimurium, and their efficacies were then compared by the disc diffusion method. Qualitative phytochemical analysis of P. maderaspatensis confirms the presence of various phytochemicals like saponins, carbohydrate, proteins, and tannins with various Rf values (0.05, 0.27, 0.40) identified by TLC. At the concentration of 100 mg/ml the P. maderaspatensis showed maximum activity (25 mm) against Staphylococcus aureus. Others showed moderate activity. The solvent (water) were used as control whereas gentamicin was used as reference for bacteria. The solvent had no effect on the microorganisms whereas gentamicin inhibited microbial growth. The minimum inhibitory concentration (MIC) of the aqueous extracts on Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhimurium were at 75 mg/ml, 25 mg/ml,25 mg/ml, 75 mg/ml, 50 mg/ml and 25 mg/ml while the minimum bacterial concentration (MBC) were at 75 mg/ml, 50 mg/ml, 25 mg/ml, 75 mg/ml, 50 mg/ml and 25 mg/ml respectively. The observed antibacterial effects were believed to be due to the presence of saponins, carbohydrate, proteins, and tannins identified in the extracts. The results apparently justified their use in the treatment of infections.


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