A simple, rapid, precise and accurate high performance liquid chromatography method was developed for simultaneous estimation of Clobetasol propionate and Fusidic acid in combined dosage form. The separation was obtained using a mobile phase consisting of acetonitrile and water in ratio of 80:20 and adjusting pH 5.0 with glacial acetic acid (10%) using Phenomenex-luna C18 (250 × 4.6 mm, 5 μm) column. The flow rate 1.0 mL min-1 and UV detection at 240 nm was employed. The retention time for Clobetasol propionate and Fusidic acid was 5.55 min and 7.48 min respectively. Linearity for Clobetasol propionate and Fusidic acid was found to be in the range of 4-12 μg/mL and 160-480 μg/mL respectively. The method was validated as per the ICH guidelines and the results were within the acceptance criteria for precision, linearity, specificity, stability of solution and robustness.