Pharmacophore an International Research Journal
Pharmacophore
Submit Manuscript
Open Access | Published: 2023 - Issue 3

Anticancer Effect of Actinomycetes Secondary Metabolite Against Breast Cancer Cell Line (MCF-7); Cytological and Molecular Studies Download PDF


, , , , ,
Abstract

Heliomycin is a natural compound extracted from the metabolites of Actinomycetes bacteria. We aim to evaluate the anticancer role of Heliomycin, against Tamoxifen as a standard drug. Flow cytometry and analysis of the cell cycle to detect the ratios of apoptosis, and cell cycle arrest were performed, and the genes expression was assayed using real-time RT-PCR. CK-19 protein was assessed using immunocytochemistry. Also, cytopathological alterations were evaluated using H&E stain and electron microscope examination. qRT- PCR showed that HER-2 expression was less elevated in tamoxifen-treated cells than in heliomycin-treated cells in the control cell. For P53 it showed significant up-regulation in heliomycin-treated cells than tamoxifen-treated cells compared to the control. In contrast, ERα, TNFα, and TLR-4 showed the reverse. The flow cytometry assay revealed a considerable increase in early and late apoptosis in the treated group with Tamox. and Helio. compared with the control. H&E stain and ultrastructure study showed cell apoptosis and chromatin degradation and cell shrinkage in treated cells against control. Immunohistochemically, CK-19 showed a severe reaction in control cells while a mild reaction in heliomycin-treated cells versus a moderate reaction in tamoxifen-treated cells. Heliomycin possesses accepted anticancer behaviour against breast cancer MCF-7 cells, as it activates cancerous cell apoptosis with upregulation of tumour suppressor genes.

Cite this article
Vancouver
Hassan BN, Azzuni ASE, Abdelfattah MS, Elgabri MS, Ahmed AS, Abdo SM. Anticancer Effect of Actinomycetes Secondary Metabolite Against Breast Cancer Cell Line (MCF-7); Cytological and Molecular Studies. Pharmacophore. 2023;14(3):23-34. https://doi.org/10.51847/jMXpFOxF3o
APA
Hassan, B. N., Azzuni, A. S. E., Abdelfattah, M. S., Elgabri, M. S., Ahmed, A. S., & Abdo, S. M. (2023). Anticancer Effect of Actinomycetes Secondary Metabolite Against Breast Cancer Cell Line (MCF-7); Cytological and Molecular Studies. Pharmacophore, 14(3), 23-34. https://doi.org/10.51847/jMXpFOxF3o

QR code:

Short Link:
Views: 3138

Downloads: 335
Quick Access

Associations

Pharmacophore
ISSN: 2229-5402

Copyright © 2024 Pharmacophore. Authors retain copyright of their article if they are accepted for publication.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.