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Open Access | Published: 2023 - Issue 3

Anticancer Effect of Actinomycetes Secondary Metabolite Against Breast Cancer Cell Line (MCF-7); Cytological and Molecular Studies Download PDF


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Abstract

Heliomycin is a natural compound extracted from the metabolites of Actinomycetes bacteria. We aim to evaluate the anticancer role of Heliomycin, against Tamoxifen as a standard drug. Flow cytometry and analysis of the cell cycle to detect the ratios of apoptosis, and cell cycle arrest were performed, and the genes expression was assayed using real-time RT-PCR. CK-19 protein was assessed using immunocytochemistry. Also, cytopathological alterations were evaluated using H&E stain and electron microscope examination. qRT- PCR showed that HER-2 expression was less elevated in tamoxifen-treated cells than in heliomycin-treated cells in the control cell. For P53 it showed significant up-regulation in heliomycin-treated cells than tamoxifen-treated cells compared to the control. In contrast, ERα, TNFα, and TLR-4 showed the reverse. The flow cytometry assay revealed a considerable increase in early and late apoptosis in the treated group with Tamox. and Helio. compared with the control. H&E stain and ultrastructure study showed cell apoptosis and chromatin degradation and cell shrinkage in treated cells against control. Immunohistochemically, CK-19 showed a severe reaction in control cells while a mild reaction in heliomycin-treated cells versus a moderate reaction in tamoxifen-treated cells. Heliomycin possesses accepted anticancer behaviour against breast cancer MCF-7 cells, as it activates cancerous cell apoptosis with upregulation of tumour suppressor genes.

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Vancouver
Hassan BN, Azzuni ASE, Abdelfattah MS, Elgabri MS, Ahmed AS, Abdo SM. Anticancer Effect of Actinomycetes Secondary Metabolite Against Breast Cancer Cell Line (MCF-7); Cytological and Molecular Studies. Pharmacophore. 2023;14(3):23-34. https://doi.org/10.51847/jMXpFOxF3o
APA
Hassan, B. N., Azzuni, A. S. E., Abdelfattah, M. S., Elgabri, M. S., Ahmed, A. S., & Abdo, S. M. (2023). Anticancer Effect of Actinomycetes Secondary Metabolite Against Breast Cancer Cell Line (MCF-7); Cytological and Molecular Studies. Pharmacophore, 14(3), 23-34. https://doi.org/10.51847/jMXpFOxF3o

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