Introduction: Datura stramonium Linn (D. stramonium L.) is an annual plant that belongs to the Solanaceae, a plant family that is poisonous and emits an unpleasant odor due to the presence of tropane alkaloids. Aim: The study was designed to determine the amount of hyoscyamine in the crude ethanol extract of Datura stramonium L. seeds. Materials and Methods: Fresh D. stramonium L. seeds were procured, identified, extracted, and fractioned in chloroform. The percentage yield was determined. The presence of alkaloids was qualitatively confirmed by phytochemical screening and thin-layer chromatography. The retention factor was determined. The high-performance liquid chromatography (HPLC) of the samples was carried out and the calibration curve was plotted to determine the amount of the hyoscyamine fraction. Results: An 11.04 µg/ml amount of hyoscyamine was obtained at the limit of detection (LOD) and quantification (LOQ) of 0.75 and 2.26 µg/ml respectively. Conclusion: In conclusion, the fraction of hyoscyamine in the D. stramonium L. seeds was little compared to the quantity of the crude seeds.
Key Messages: The extraction and fractionation of hyoscyamine from the crude ethanol extract of Datura stramonium Linn seeds reveal the presence of several phytoconstituents including alkaloids. The hyoscyamine yield was little when compared to the crude sample of the seed used.