Pharmacophore an International Research Journal
The current work's objective was to examine the cytotoxic activity of recently created peptide esters of Galantamine: GAL-LEU and GAL-VAL on prostate cancer PC3 cell lines. For the estimation of the cytotoxic effect of Galantamine derivatives, the MTT reduction assay was applied. PC3 cells were triplicate exposed separately to each of the peptide esters, applied in different concentrations (1.875 mМ ÷ 30 mМ). In the MTT test, the reduction of tetrazolium salt MTT resulted in the creation of formazan, whose absorbance was measured spectrophotometrically at wavelength 570 nm. The experimental results show that peptide ester GAL-LEU at 30 mM inhibits 55.36% of PC3 cell growth with an index of cell viability of 44.64 %. The lower antiproliferative effect of derivative GAL-VAL was proven by the fact that 30 mM inhibits 43.96 % of cell growth. The results showed that both of the tested esters had cytotoxic action against the PC3 cell line, but that GAL-LEU has a stronger antiproliferative impact than GAL-VAL (IC50 >30 mM), because of its lower value of IC50 = 30.8 mM.
