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Open Access | Published: 2023 - Issue 5

Isolation and Purification of Superoxide-Producing Protein Complex from Helianthus tuberosus, Daucus sativus, and Solanum tuberosum Download PDF


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Abstract

In this study, isoforms of NPC-Fe(III) complexes were isolated and purified from Armenian Helianthus tuberosus, Solanum tuberosum, and Daucus sativus. The physicochemical properties of these complexes were determined for the first time, including specific amounts, О2--producing activity, stationary concentration of О2-, and fluorescence intensity. The complexes exhibit maximal optical absorptions in the visible and UV regions. Hybrid associates between the isolated NPC-Fe(III) complexes and NADPH oxidase (Nox) were formed, and the production of О2- by these hybrid associates was observed. The complexes continuously produce О2- in aerobic conditions by utilizing electrons from NPC, transferring them to Fe(III), and subsequently reducing О2 to О2-. Finally, О2 stabilizes О2-.

Overall, these results suggest that the isoforms of NPC-Fe(III) complexes represent new prooxidant components in the aforementioned sources and that NPC serves as a bioelectric  source for the production of О2-.

 

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Vancouver
Simonyan R, Babayan M, Yekmalyan H, Alexanyan A, Simonyan G, Alexanyan S, et al. Isolation and Purification of Superoxide-Producing Protein Complex from Helianthus tuberosus, Daucus sativus, and Solanum tuberosum. Pharmacophore. 2023;14(5):107-11. https://doi.org/10.51847/XdwSHDmxQr
APA
Simonyan, R., Babayan, M., Yekmalyan, H., Alexanyan, A., Simonyan, G., Alexanyan, S., Darbinyan, L., Simonyan, K., & Simonyan, M. (2023). Isolation and Purification of Superoxide-Producing Protein Complex from Helianthus tuberosus, Daucus sativus, and Solanum tuberosum. Pharmacophore, 14(5), 107-111. https://doi.org/10.51847/XdwSHDmxQr

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