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Open Access | Published: 2024 - Issue 5

Hibiscus sabdariffa Leaf Extract Activates Molecular Gene Expressions of Insulin and GLP-I Receptor in Streptozotocin-Induced Rats Download PDF


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  1. Phytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria.

  2. Department of Environmental Management and Toxicology, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria.
  3. Medical Biochemistry Unit, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Nigeria.
  4. Biochemical Immunology and Phytomedicine Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria.
  5. Phytomedicine, Functional Food Biochemistry and Toxicology, Department of Biochemistry, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria.
  6. Institute of Drug Research and Development, SE Bogoro Center, Afe Babalola University, Ado-Ekiti, Nigeria
  7. Phytomedicine, Biochemical Toxicology and Biotechnology Research Laboratories, Department of Biochemistry, College of Sciences, Afe Babalola University, Ado-Ekiti, Nigeria.
  8. Biotechnology and Structural Biology (BSB) Group, Department of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa, South Africa.
  9. Department of Biomedical, Surgical and Dental Sciences, Milan State University, Milan, Italy.
Abstract

This work investigated the therapeutic effects of flavonoid-rich extracts obtained from Hibiscus sabdariffa L. leaves in streptozotocin-induced diabetic rats. Their biochemical parameters such as redox stress biomarkers, anti-inflammatory levels, pancreatic histology, insulin concentration, lipase activity, purinergic enzyme activities, homeostasis model assessment of β-cells (HOMA-β), and homeostasis model assessment of insulin resistance (HOMA-IR) were assessed. Furthermore, the relative gene expressions of insulin receptors and GLP-1 were examined. H. sabdariffa extracts significantly (p<0.05) mitigated redox stress and inflammation in diabetic rats, as indicated by the reduction in malondialdehyde (MDA) levels, interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and C-reactive protein (CRP) as well as enhancement of reduced glutathione (GSH), glutathione-S-transferase (GST), catalase (CAT), and glutathione peroxidase (GPx). Pancreatic histological analysis revealed that the extracts protect against the degeneration of pancreatic acinar cells and beta cells. Moreover, they enhance pancreatic insulin concentration and reduce lipase activity in diabetic rats. In addition, the extracts positively affected purinergic enzyme activities, specifically ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) and ATPase besides significantly improving the homeostasis model assessments of β-cells and insulin resistance. Most notably, diabetic rats treated with H. sabdariffa leaf extracts activated the relative gene expressions of insulin and GLP-1 receptors. Hence, these findings support the potential of H. sabdariffa leaf extracts as a natural therapeutic agent for managing diabetes.

Cite this article
Vancouver
Ajiboye BO, Famusiwa CD, Ojelabi AO, Akinola WO, Adeyemi ET, Adejumo AA, et al. Hibiscus sabdariffa Leaf Extract Activates Molecular Gene Expressions of Insulin and GLP-I Receptor in Streptozotocin-Induced Rats. Pharmacophore. 2024;15(5):15-26. https://doi.org/10.51847/xSFzqq25OY
APA
Ajiboye, B. O., Famusiwa, C. D., Ojelabi, A. O., Akinola, W. O., Adeyemi, E. T., Adejumo, A. A., Owolabi, O. V., Jeje, T. O., Bamisaye, F. A., Oyinloye, B. E., et al. (2024). Hibiscus sabdariffa Leaf Extract Activates Molecular Gene Expressions of Insulin and GLP-I Receptor in Streptozotocin-Induced Rats. Pharmacophore, 15(5), 15-26. https://doi.org/10.51847/xSFzqq25OY

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